Fast Cell Processing
- Reduced buffer consumption for improved efficiency
- Minimized dwell time outside the bioreactor
Nucleic Acids
Achieve high-yield plasmid DNA production with low-shear centrifuge technology designed for efficient harvesting and clarification. UniFuge®, ViaFuge®, and PowerFuge® support scalable workflows from research to large-scale manufacturing.
How It Works
Streamlined centrifuge solutions for scalable plasmid DNA production
Tubular bowl centrifuge technology provides a low-shear solution for plasmid DNA workflows, supporting plasmid purification while maintaining the integrity of large, shear-sensitive molecules. From harvesting high-density cultures to post-lysis clarification, these centrifuges streamline key processes while reducing contamination risks with closed-system operation.
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Cell BankingThe closed system harvests and concentrates cells, performing media exchange to reduce contamination risk during long-term storage. |
Cell HarvestLow-shear separation gently concentrates cells from high-density cultures, minimizing damage and preserving viability. |
ConcentrationEfficiently reduces culture volume while maintaining the integrity of cells and plasmid DNA, optimizing workflows for downstream processing. |
Cell WashingGentle washing removes residual media and impurities, preparing cells for critical extraction steps. |
ResuspensionResuspends cells in optimized buffers to maintain cell and plasmid DNA integrity, enabling consistent downstream applications. |
Post-Lysis ClarificationLow-shear separation removes cell debris after lysis, ensuring high recovery of plasmid DNA with minimal impurities. |
Key Benefits
Benefits of using CARR Biosystems for Nucleic Acids
Low-Shear Handling
- Low-shear tubular bowl technology minimizes cfDNA damage
- Gentle handling preserves sensitive molecules
Enhanced Workflow Efficiency
- Automated buffer exchange reduces manual intervention
- Resuspension accelerates downstream processing
Seamless Scalability
- Seamless scalability from development to manufacturing
- Adaptable from 1L culture preparations to tens of liters
- 21 CFR Part 11 compliant
Endorsements
See what people are saying about Nucleic Acids
“This really opens the door with the potential to go above 300 L.”
mRNA and pDNA Bioproduction Expert
“The high g-force of the PowerFuge [e. Coli lysate clarification] reduced our downstream clarification needs.”
Senior Process Development Scientist, pDNA
Centrifuges
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Frequently Asked Questions (FAQs) About Nucleic Acids
Is the single-use UniFuge capable of removing E. coli from solution?
Yes, the UniFuge can efficiently separate E. coli from solution using high relative centrifugal forces (RCF or XG). This capability ensures robust removal of bacterial cells, even at high densities, making it ideal for workflows requiring efficient bacterial separation.
How do CARR centrifuges achieve low-shear processing for plasmid DNA?
Centrifuges use advanced tubular bowl technology, which minimizes shear forces during processing. This gentle handling is especially crucial for plasmid DNA post-lysis, when it becomes cell-free and highly sensitive to shear damage. By preserving the integrity of large plasmid DNA molecules, this technology supports efficient plasmid purification and consistent yields. The low-shear design is ideal for workflows where maintaining the quality of shear-sensitive biomolecules is critical.
Can CARR centrifuges handle washing and resuspension steps in plasmid DNA production?
Yes, our centrifuges simplify washing and resuspension by automating these steps in a closed system. This reduces contamination risks, minimizes manual intervention, and accelerates the preparation of cells for downstream processes, such as lysis or buffer exchange.
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